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KMID : 1134820070360081055
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 8 p.1055 ~ p.1061
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Effects of Gamma Irradiation on Color Changes and Antioxidative Activities of Caesalpinia sappan L.
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Kwon Hyung-Jun
Jung U-Hee
Park Hae-Ran
Shin Dong-Hwa
Jo Sung-Kee
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Abstract
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Caesalpinia sappan L. is an indeciduous tree distributed in China, India, Miyanmar and Vietnam. Its heartwood has long been used in oriental folk medicines to treat diseases. In this study, antioxidative of Caesalpinia sappan L. and the effect of gamma irradiation on its chemical and biological properties were investigated. The ethyl acetate fraction (EtOAc fr.) of Caesalpinia sappan L. was irradiated with 100 kGy of gamma ray. The dark red color of EtOAc fr. was significantly (p＜0.05) removed by irradiation (Hunter L and b values increased and a value decreased). The total phenolic content of EtOAc fr. was 865 ㎎/g and it was increased to 1195 ㎎/g by gamma irradiation. DPPH radical and superoxide anion radical scavenging activities and lipid peroxidation inhibitory activity of EtOAc fr. were very high and its activities were also increased by gamma irradiation. EtOAc fr. also inhibited the irradiation?induced DNA damage of lymphocyte as determined by comet assay. In conclusion, EtOAc fr. of Caesalpinia sappan L. extract showed high antioxidative activities in vitro. Furthermore, gamma irradiation on EtOAc fr. ameliorated the color and antioxidative properties. Therefore, it can be suggested that Caesalpinia sappan L. may be a good material for antioxidant function and gamma irradiation may be applied for the improvement of chemical and biological properties of Caesalpinia sappan L.
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KEYWORD
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Caesalpinia sappan L., color, gamma irradiation, antioxidative activities
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